The present study deals with effect of chemical mutagen, Ethyl Methane Sulphonate on seeds of tomato (Lycopersicumesculentum Mill.). Tomato is used as vegetable all over the world. Besides its use in cooking it has got some medicinal properties also. It is a very rich source of vitamin C. It is supposed to help in developing immune system of the body. Five different concentrations of the Ethyl Methane Sulphonate were applied as 0.10 percent, 0.20 percent, 0.30 percent, 0.40 percent and 0.50 percent. The effect of Ethyl Methane Sulphonate can stimulate higher germination rate.